The anxiolytic effect of CBD on chronically stressed mice depends on hippocampal neurogenesis: involvement of the endocannabinoid system.
Cannabidiol (CBD), the main non-psychotomimetic component of the plant Cannabis sativa, exerts therapeutically promising effects on human mental health such as inhibition of psychosis, anxiety and depression. However, the mechanistic bases of CBD action are unclear. Here we investigate the potential involvement of hippocampal neurogenesis in the anxiolytic effect of CBD in mice subjected to 14 d chronic unpredictable stress (CUS). Repeated administration of CBD (30 mg/kg i.p., 2 h after each daily stressor) increased hippocampal progenitor proliferation and neurogenesis in wild-type mice. Ganciclovir administration to GFAP-thymidine kinase (GFAP-TK) transgenic mice, which express thymidine kinase in adult neural progenitor cells, abrogated CBD-induced hippocampal neurogenesis. CBD administration prevented the anxiogenic effect of CUS in wild type but not in GFAP-TK mice as evidenced in the novelty suppressed feeding test and the elevated plus maze. This anxiolytic effect of CBD involved the participation of the CB1 cannabinoid receptor, as CBD administration increased hippocampal anandamide levels and administration of the CB1-selective antagonist AM251 prevented CBD actions. Studies conducted with hippocampal progenitor cells in culture showed that CBD promotes progenitor proliferation and cell cycle progression and mimics the proliferative effect of CB1 and CB2 cannabinoid receptor activation. Moreover, antagonists of these two receptors or endocannabinoid depletion by fatty acid amide hydrolase overexpression prevented CBD-induced cell proliferation. These findings support that the anxiolytic effect of chronic CBD administration in stressed mice depends on its proneurogenic action in the adult hippocampus by facilitating endocannabinoid-mediated signalling.
Read the study here
....This information is presented for educational purposes only. 1Pure provides this information to give an understanding of the potential applications of cannabidiol. Links to third party websites do not constitute an endorsement of these organizations by 1Pure and none should be inferred. ..Esta informação é apresentada apenas para fins educacionais. 1Pure fornece informações para dar uma compreensão das aplicações potenciais do canabidiol. Links para sites de terceiros não constituem um endosso dessas organizações por parte da 1Pure e nada deve ser inferido. ..1Pure proporciona información para dar una comprensión de las aplicaciones potenciales del canabidiol. Los enlaces a sitios de terceros no constituyen un endoso de estas organizaciones por parte de 1Pure y no se debe inferir nada. ....